In South Africa, the Limpopo Province houses 63 wastewater treatment plants, and findings of the Green Drop assessment indicated that most of these plants are in the high risk and critical risk space, except for four plants, which are still in the low and medium risk space. The current study, therefore, investigated the presence of Vibrio cholerae and its biotypes/ serotypes in selected wastewater treatment plants (WWTP) of the Limpopo Province (Burgersfort Wastewater Treatment Plant, Paarl Wastewater Treatment Plant, Thohoyandou Wastewater Treatment Plant, Makhado Wastewater Treatment Plant, and Ga-Kgapane Wastewater Treatment Plant). The selection was based on their non-compliance between 2009 and 2013 as reported in the Green Drop statistics by the Department of Water and Sanitation.
